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. 2007 Aug 6;27(20):7143–7160. doi: 10.1128/MCB.00253-07

FIG. 1.

FIG. 1.

GRα induced apoptosis in U-2 OS cells. (A) Cells stably expressing hGRα were subjected to Western blot analysis. These cells express GR-A, -B, -C, and -D isoforms as indicated. (B) When treated with vehicle or DEX (100 nM) for 48 h, nucleus staining showed chromatin condensation in DEX-treated cells containing GR but not in those lacking GR. (C) Flow cytometric analysis of PI labeling showed that a higher percentage of cells expressing GR were PI positive, as indicated in the histogram highlighted with an arrow. (D) Annexin V labeling showed that GR-expressing cells, when treated with DEX, had a greater number of cells positive for cell surface annexin V and negative for PI, as indicated in the lower right quadrants on the dot plots. (E) The active PARP level was increased in GR-expressing cells as determined by Western blot analysis. (F) DNA degradation was induced in GR-expressing cells as indicated by arrows on the histogram. (G) Caspase activity was elevated in GR-expressing cells, as indicated by arrows on the histogram.