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. 2007 Aug 6;27(20):7143–7160. doi: 10.1128/MCB.00253-07

FIG. 2.

FIG. 2.

Stable expression of GR isoforms in U-2 OS cells. (A) Individual GRα isoforms were expressed at comparable levels in the absence and presence of DEX (100 nM for 2 h) as determined by Western blot analysis and ligand binding assay. Additional bands between the GR-C and -D isoforms may be nonspecific signals, and they were not consistently detected. Bmax and Kd values ± standard errors of the means (SEM) are shown. (B) The half-lives of each GR isoform were similar. The expression of each GR isoform was turned off by doxycycline. The GR protein level was determined in the absence or presence of DEX (100 nM) by Western blot analysis.