FIG. 9.

Combination of GR isoforms. (A) Three U-2 OS cell lines were generated, where the level of the GR-A isoform is constant and the level of the second GR isoform can be regulated by addition of doxycycline. (B) The constitutively expressed GR-A isoform is responsible for 20 to 30% of cell death (0.3 ng/ml doxycycline). Increases in the amount of the GR-A (control) or the GR-C isoform induced additional cell death as measured by PI staining, whereas increases in the amount of the GR-D isoform did not.