FIG. 4.
Herp2 interacts with the E-I element of p57Kip2 promoter in yeast. (A) Diagram of the mouse p57Kip2 gene showing the conserved elements E-I and E-II (black boxes). E-I and E-II were identified through sequence comparison among human, rat, and mouse p57KIP2 genes. Empty boxes represent exons. (B) Herp2 interacts with only E-I. Neither E-II nor the p53-binding site interacts with Herp2. The interaction was abolished by the deletion of the N-terminal region of Herp2, which contains the DNA-binding domain. (C) Mapping of the Herp2-binding site within E-I. (D) The E box in E-I-5 is essential for interaction with Herp2. Mutating the E box destroyed the ability of E-I-5 to interact with Herp2.