FIG. 1.

Schematic representation of N-terminal mutagenesis of LcrV. The Frame +1, Frame −1, and Scramble mutants are shown in panel A, while the in-frame deletion mutants V1 to V17 are displayed in panel B. All mutants were introduced in cis on the Ysc-Yop virulence plasmid (pIB102) to avoid any copy number effects. (A) Numbers 1 to 20 indicate the nucleotide triplets positioned with respect to the start codon of LcrV. Altered or inserted residues are shaded in gray. (B) Residues missing in the deletion mutants are indicated within parentheses and are illustrated by broken lines. Mutant V11 (Δ25-40) was not included.