TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant genotype or phenotype | Source or reference |
|---|---|---|
| Strains | ||
| E. coli strains | ||
| Top10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 deoR araD139 Δ(ara-leu)7679 galU galK rpsL (Strr) endA1 nupG | Invitrogen |
| S17-1 λpir | recA thi pro hsdRM+ Smr <RP4:2-Tc:Mu:Ku:Tn7> Tpr | 48 |
| Y. pseudotuberculosis strains | ||
| YPIII/pIB102 | yadA::Tn5 (wild type) Kmr | 5 |
| YPIII/pIB10201 | pIB102; lcrV with a +1 frameshift mutation incorporating codons 4 to 13; Kmr | This study |
| YPIII/pIB10202 | pIB102; lcrV with a −1 frameshift mutation incorporating codons 2 to 15; Kmr | This study |
| YPIII/pIB10203 | pIB102; lcrV with wobble base mutations between codons 1 and 15, altering only the mRNA sequence (Scramble); Kmr | This study |
| YPIII/pIB10204 | pIB102; lcrV in-frame deletion of codons 2 to 20; Kmr | This study |
| YPIII/pIB10205 | pIB102; lcrV in-frame deletion of codons 3 to 20; Kmr | This study |
| YPIII/pIB10206 | pIB102; lcrV in-frame deletion of codons 5 to 20; Kmr | This study |
| YPIII/pIB10207 | pIB102; lcrV in-frame deletion of codons 7 to 20; Kmr | This study |
| YPIII/pIB10208 | pIB102; lcrV in-frame deletion of codons 9 to 20; Kmr | This study |
| YPIII/pIB10209 | pIB102; lcrV in-frame deletion of codons 11 to 20; Kmr | This study |
| YPIII/pIB10210 | pIB102; lcrV in-frame deletion of codons 13 to 20; Kmr | This study |
| YPIII/pIB10211 | pIB102; lcrV in-frame deletion of codons 15 to 20; Kmr | This study |
| YPIII/pIB10212 | pIB102; lcrV in-frame deletion of codons 17 to 20; Kmr | This study |
| YPIII/pIB10213 | pIB102; lcrV in-frame deletion of codons 19 and 20; Kmr | This study |
| YPIII/pIB10214 | pIB102; lcrV in-frame deletion of codons 25 to 40; Kmr | This study |
| YPIII/pIB10215 | pIB102; lcrV in-frame deletion of codons 2 to 4; Kmr | This study |
| YPIII/pIB10216 | pIB102; lcrV in-frame deletion of codons 5 to 7; Kmr | This study |
| YPIII/pIB10217 | pIB102; lcrV in-frame deletion of codons 8 to 10; Kmr | This study |
| YPIII/pIB10218 | pIB102; lcrV in-frame deletion of codons 11 to 13; Kmr | This study |
| YPIII/pIB10219 | pIB102; lcrV in-frame deletion of codons 14 to 16; Kmr | This study |
| YPIII/pIB10220 | pIB102; lcrV in-frame deletion of codons 17 to 18; Kmr | This study |
| YPIII/pIB19 | pIB102; lcrV in-frame deletion of codons 10 to 313; Kmr | 39 |
| YPIII | Virulence plasmid-cured strain | 4 |
| YPIII/pIB26 | pIB102; lcrQ in-frame deletion; Kmr Spr/Smr | 40 |
| YPIII/pIB1926 | pIB19; lcrQ in-frame deletion; Kmr Spr/Smr | 19 |
| YPIII/pIB1020126 | pIB10201; in-frame deletion of lcrQ; Kmr Spr/Smr | This study |
| YPIII/pIB1020226 | pIB10202; in-frame deletion of lcrQ; Kmr Spr/Smr | This study |
| YPIII/pIB1020326 | pIB10203; in-frame deletion of lcrQ; Kmr Spr/Smr | This study |
| YPIII/pIB1020426 | pIB10204; in-frame deletion of lcrQ; Kmr Spr/Smr | This study |
| YPIII/pIB1020526 | pIB10205; in-frame deletion of lcrQ; Kmr Spr/Smr | This study |
| YPIII/pIB1020626 | pIB10206; in-frame deletion of lcrQ; Kmr Spr/Smr | This study |
| YPIII/pIB1020726 | pIB10207; in-frame deletion of lcrQ; Kmr Spr/Smr | This study |
| YPIII/pIB1020826 | pIB10208; in-frame deletion of lcrQ; Kmr Spr/Smr | This study |
| YPIII/pIB1020926 | pIB10209; in-frame deletion of lcrQ; Kmr Spr/Smr | This study |
| YPIII/pIB1021026 | pIB10210; in-frame deletion of lcrQ; Kmr Spr/Smr | This study |
| YPIII/pIB1021126 | pIB10211; in-frame deletion of lcrQ; Kmr Spr/Smr | This study |
| YPIII/pIB1021226 | pIB10212; in-frame deletion of lcrQ; Kmr Spr/Smr | This study |
| YPIII/pIB1021326 | pIB10213; in-frame deletion of lcrQ; Kmr Spr/Smr | This study |
| YPIII/pIB1021426 | pIB10214; in-frame deletion of lcrQ; Kmr Spr/Smr | This study |
| YPIII/pIB1021526 | pIB10215; in-frame deletion of lcrQ; Kmr Spr/Smr | This study |
| YPIII/pIB1021626 | pIB10216; in-frame deletion of lcrQ; Kmr Spr/Smr | This study |
| YPIII/pIB1021726 | pIB10217; in-frame deletion of lcrQ; Kmr Spr/Smr | This study |
| YPIII/pIB1021826 | pIB10218; in-frame deletion of lcrQ; Kmr Spr/Smr | This study |
| YPIII/pIB1021926 | pIB10219; in-frame deletion of lcrQ; Kmr Spr/Smr | This study |
| YPIII/pIB1022026 | pIB10220; in-frame deletion of lcrQ; Kmr Spr/Smr | This study |
| Plasmids | ||
| pCR4-TOPO | TA cloning vector; Kmr Ampr | Invitrogen |
| pDM4 | Suicide plasmid carrying sacBR; Cmr | 31 |
| pRN53 | pDM4 in which the entire lcrQ allele is replaced by an Sp/Sm resistance gene; Cmr Spr/Smr | 40 |
| pJEB357 | pDM4 encoding LcrVΔ2-20; Cmr | This study |
| pJEB358 | pDM4 encoding LcrVΔ3-20; Cmr | This study |
| pJEB359 | pDM4 encoding LcrVΔ5-20; Cmr | This study |
| pJEB360 | pDM4 encoding LcrVΔ7-20; Cmr | This study |
| pJEB361 | pDM4 encoding LcrVΔ9-20; Cmr | This study |
| pJEB362 | pDM4 encoding LcrVΔ11-20; Cmr | This study |
| pJEB363 | pDM4 encoding LcrVΔ13-20; Cmr | This study |
| pJEB364 | pDM4 encoding LcrVΔ15-20; Cmr | This study |
| pJEB365 | pDM4 encoding LcrVΔ17-20; Cmr | This study |
| pJEB366 | pDM4 encoding LcrVΔ19-20; Cmr | This study |
| pJEB367 | pDM4 encoding LcrVΔ25-40; Cmr | This study |
| pJEB368 | pDM4 encoding LcrV with a +1 frameshift mutation incorporating codons 4 to 13; Cmr | This study |
| pJEB369 | pDM4 encoding LcrV with a −1 frameshift mutation incorporating codons 2 to 15; Cmr | This study |
| pJEB370 | pDM4 encoding LcrV with wobble base mutations of codons 2 to 15; Cmr | This study |
| pJEB378 | pDM4 encoding LcrVΔ2-4; Cmr | This study |
| pJEB379 | pDM4 encoding LcrVΔ5-7; Cmr | This study |
| pJEB380 | pDM4 encoding LcrVΔ8-10; Cmr | This study |
| pJEB381 | pDM4 encoding LcrVΔ11-13; Cmr | This study |
| pJEB382 | pDM4 encoding LcrVΔ14-16; Cmr | This study |
| pJEB383 | pDM4 encoding LcrVΔ17-18; Cmr | This study |