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. 2007 Sep 14;189(23):8417–8429. doi: 10.1128/JB.00936-07

TABLE 3.

lcrV mutants in cis and their phenotypesa

LcrV variant LcrV phenotype
Yop phenotype
Growth phenotyped lcrQ::sp-sm effect
Translocatione
Stabilityb Synthesisc Secretionc Synthesisc Secretionc LcrV secretionc Yop secretionc
Parent ↓ ↓ ↓ ↓ ↓ CD ↑ (plus Ca2+) ↓ ↓
LcrVnull NA NA NA ↓↓ ↓↓ CI NA ↓ ↓↓
Frame +1 ↔ ↔ ↔ ↔ ↔ CD ↑ (plus Ca2+) ↔ ↔
Frame −1 ↔ ↔ ↔ ↔ ↔ CD ↑ (plus Ca2+) ↔ ↔
Scramble ↔ ↔ ↔ ↔ ↔ CD ↑ (plus Ca2+) ↔ ↔
V1 (Δ2-20) ↓ ↔ ↓↓g ↓ ↓ CD-like ↓↓g ↔ ↓↓
V2 (Δ3-20) ↔ ↔ ↓↓g ↓ ↓ CD-like ↓↓g ↔ ↓↓
V3 (Δ5-20) ↔ ↑ ↓↓g ↓ ↓ CD-like ↓↓g ↑ (plus Ca2+) ↓↓
V4 (Δ7-20) ↔ ↑ ↓↓g ↓ ↓ CD-like ↓↓g ↔ ↓↓
V5 (Δ9-20) ↔ ↔ ↓↓g ↓ ↓ CD-like ↓↓g ↔ ↓↓
V6 (Δ11-20) ↔ ↔ ↓↓g ↓ ↓ CD-like ↓↓g ↔ ↓↓
V7 (Δ13-20) ↔ ↔ ↓↓g ↓ ↓ CD-like ↓↓g ↑ (plus Ca2+) ↓↓
V8 (Δ15-20) ↔ ↔ ↓ ↓ ↓ CD-like ↑ (plus Ca2+) ↔ ↔
V9 (Δ17-20) ↔ ↔ ↓ ↓ ↓ CD ↑ (plus Ca2+) ↑ (translocators)f ↔
V10 (Δ19-20) ↔ ↔ ↓ ↔ ↔ CD ↑ (plus Ca2+) ↔ ↔
V11 (Δ25-40) ↔ ↔ ↓ ↓ ↓ CD ↑ (plus Ca2+) ↔ ↔
V12 (Δ2-4) ↔ ↔ ↓↓g ↓ ↓ CD-like ↓↓g ↔ ↓↓
V13 (Δ5-7) ↔ ↔ ↓ ↔ ↔ CD ↑ (plus Ca2+) ↑ (translocators)f ↔
V14 (Δ8-10) ↔ ↔ ↓ ↓ ↓ CD ↑ (plus Ca2+) ↔ ↔
V15 (Δ11-13) ↔ ↔ ↓↓g ↓ ↓ CD ↓↓g ↔ ↓↓
V16 (Δ14-16) ↔ ↔ ↔ ↔ ↔ CD ↔ ↔ ↔
V17 (Δ17-18) ↔ ↔ ↔ ↔ ↔ CD ↔ ↔ ↔
a

↑, the mutant achieved a better functional level than did the parent; ↔, the mutant is functionally equivalent to the parent; ↓, the mutant partially lost function compared to the parent; ↓↓, the mutant has a severe phenotypic defect; NA, not available.

b

Protein stability was measured by sensitivity to endogenous proteases (37).

c

Analysis of Yop production and secretion was assessed following growth in BHI medium without Ca2+ (T3S inducing) or with Ca2+ (T3S repressing).

d

The Yersinia growth phenotype was calculated by monitoring growth at 37°C in TMH medium alone (minus Ca2+) or supplemented with 2.5 mM CaCl2 (plus Ca2+) (57).

e

The YopE-dependent HeLa cell cytotoxicity assay measured the extent of altered cell monolayer morphology (46).

f

In an lcrQ background, these lcrV mutants selectively secreted translocator proteins constitutively.

g

Loss of LcrV secretion.