FIG. 1.
A. Arrangement of the yydFGHIJ operon. The schematic is drawn to scale, and predicted functions are shown above the genes. Large arrows represent the open reading frames, with the PyydF and PyydI promoters identified in this study marked. Open triangles show the positions of the Tn7 transposon insertions: 1, YPL3, YPL9, YPL14, and YPL15; 2, YPL6 and YPL13. Also indicated are the positions of the fragments used by Albano et al. for gel shift and pMUTIN construction (1) and the fragments used in this study to create lacZ fusions at the amyE locus. B. The yydF promoter region and yydF-yydG intergenic region. The −35 and −10 regions of a σA-type promoter are underlined, and the +1 start of transcription as determined by 5′-RACE PCR is shown in bold. The yydF and yydG open reading frames are indicated by dashes, and the inverted repeat is shown with arrows (with the complementary bases marked in bold).