TABLE 4.
Properties and functions of the proteins identified during Tn7 mutagenesis and selection for upregulation of PliaI
| Protein | Properties | Conserved domain(s)c | Closest homologd (%identity/%positive) | Function |
|---|---|---|---|---|
| YydF | 49 aa, +2.2 charge at pH 7.0, 34% hydrophobic, potential to form α-helix with 12 hydrophobic residues on the same surfacea | None | Streptococcus agalactiae strains (46-50/69) | Antimicrobial/signal/secreted peptidee |
| YydG | 319 aa, cytoplasmic protein | Predicted Fe-S oxidoreductase (COG0535), with 5′ region radical SAM superfamily (pfam 04055) | Streptococcus agalactiae COH1 (35/55) | Oxidoreductasee |
| YydH | 252 aa, 5 THMb | None | Streptococcus agalactiae COH1 (34/54) | Peptidasee (M50 peptidase familyf) |
| YydI | 209 aa, cytoplasmic protein | ABC_ATPase (cd00267) | Staphylococcus aureus subsp. aureus (61/77) | ABC transporter (ATPase subunit)e |
| YydJ | 240 aa, 6 THM2 | None | Staphylococcus aureus subsp. aureus (62/82) | ABC transporter (permease subunit)e |
| MutS | 858 aa | COG0249, MutS family | B. licheniformis (83/89) | DNA mismatch repair ATPase |
| MutL | 627 aa | COG0323, MutL | B. licheniformis (78/85) | DNA mismatch repair enzyme (predicted ATPase) |
a
Baesd on information in the Antimicrobial Peptide Database (http://aps.unmc.edu/AP/main.html).
b
THM, transmembrane helices, as determined using TMHMM (www.cbs.dtu.dk/services/TMHMM/) and TMPred (www.ch.embnet.org/software/TMPRED_form.html).
c
Based on the Conserved Domains database (http://www.ncbi.nlm.nih.gov/Structure/cdd/cdd.shtml).
d
Analysis with BLAST (http://www.ncbi.nlm.nih.gov/BLAST/).
e
Function predicted from sequence homology.
f
Based on information in MEROPS, the peptidase database (http://merops.sanger.ac.uk/).