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Differential expression of flu mRNA in cells containing WT or H107A σ32. The mRNA was first reverse transcribed as indicated in Materials and Methods and then amplified with flu- or 16S rRNA-specific primers (the latter for normalization) for the numbers of cycles indicated. Only the observed bands after 19 and 22 cycles were used to calculate the relative amounts of flu mRNA in cells with WT σ32 and H107A σ32.
