FIG. 7.

Properties of CAG9301 cells expressing H107A σ³² are consistent with overexpression of the Flu protein. All cells were grown at 30°C in the absence of any added IPTG. (A) Increased sedimentation due to aggregation of cells containing the H107A σ³² gene. After the cells had been grown to an OD₆₀₀ of about 0.8, agitation was ceased. Sedimentation was determined from the decrease in the OD₆₀₀ of the upper layer of the cultures. (B) Increased surface adhesion of cells containing the H107A σ³² gene. Cells grown to an OD₆₀₀ of 0.7 to 0.8 were passed over a bed of sand, and the adhesion was determined from the reduction in the OD₆₀₀ of the effluent. The results are averages of three independent experiments. The observed difference between WT and H107A σ³² was found to be significant with a P value of 0.007 in a paired t test.