FIG. 6.

Effect of a CcpA⁻ mutant on mga expression. (A) GusA reporter assay for the M6 GAS Pmga-gusA reporter strain KSM310 (WT), the mga-inactivated derivative KSM310.150Lg (Mga⁻), and the ccpA-inactivated derivative KSM310.700 (CcpA⁻). Data are reported in GusA units (OD₄₂₀/concentration total protein [in micrograms per microliter]) and represent an average of the results from at least three independent experiments. The error bars express the standard deviations for each strain measured. (B) Mga protein production was assessed using Western analysis on whole-cell protein extracts derived from the same samples used for panel A and probed with an anti-M6 Mga antibody.