FIG. 3.

Western blot analysis of different cell extracts from wild-type S. mutans grown under anaerobic (AN) or aerobic (AE) conditions, i.e., bead-beaten SDS boiling extract (whole-cell lysates, A), 4% SDS extracts (B), bead-beaten Tris extracts (soluble fraction, C), the insoluble fraction (D), and the supernatant fraction (E). See Materials and Methods for more details about the preparation of the cellular fractions. Following SDS-PAGE, proteins were blotted onto a nitrocellulose membrane and subjected to Western blotting with an anti-GtfB polyclonal antiserum at a 1:500 dilution.