TABLE 2.
Primers used for amplification of putative promoters for cat fusions and for real-time PCR in this study
| Application and name | Sequence | Promoter or amplicon | Primer name | Sequence |
|---|---|---|---|---|
| Amplification of putative promoters | ||||
| PgtfB-5 | ATGGATCCGACAATTGTGGTGGGTAC | PgtfB | PgtfB-3 | CGCTGCAGCTTGTCCATTAGGAACCTCC |
| PgtfC-5 | GTGAATTCGATGCTAACTCTGGAGAACG | PgtfC | PgtfC-3 | GAGGATCCAAAAATAGTTAGAGTTAGTG |
| Real-time RT-PCR | ||||
| gtfB-sense | AGCAATGCAGCCATCTACAAAT | gtfB | gtfB-antisense | ACGAACTTTGCCGTTATTGTCA |
| gtfC-sense | GGTTTAACGTCAAAATTAGCTGTATTAGC | gtfC | gtfC-antisense | CTCAACCAACCGCCACTGTT |
| SMU.150-sense | GGAAGGTATCGGGTGGAGAAGC | SMU.150 | SMU.150-antisense | GAGTCGCACCTGCCAGTCC |
| SMU.299-sense | GGAGCTAATGGCTATGCTTACCG | SMU.299 | SMU.299-antisense | GCACCTGAAGCCCAGCTATTTAC |
| SMU.423-sense | CAACTGTTGAGGGTGGTGGTATG | SMU.423 | SMU.423-antisense | AAGCCGCTCCAGATACTGTACC |
| SMU.575-sense | TTGCCTAAAGCCTTACCGATTCC | SMU.575 | SMU.575-antisense | GCCTGATGGGACAAACATAAAGC |
| SMU.577-sense | TCCATCTACTTCGCAGGTCATTG | SMU.577 | SMU.577-antisense | AATCCCAGCCAGTAAAGAAACCG |
| SMU.879-sense | TTCAGAGCCTGGTTCTTCTTTCC | SMU.879 | SMU.879-antisense | TTCCCAAAGCACTGCCAACTG |
| SMU.1175-sense | GAGAAGTCTCTGGCGGACCTATG | SMU.1175 | SMU.1175-antisense | GCCACTAAGATACCTGCCAATGC |
| SMU.1508-sense | AGGAGAGATGTCTGTTGAGAAGC | SMU.1508 | SMU.1508-antisense | AACGGTTCACCTCCAGTAACAC |
| SMU.1609-sense | TGCAGCCTCAAAAGAATCCTAGC | SMU.1609 | SMU.1609-antisense | GCAATCGCAAGCCAAAAGAAGAC |
| SMU.1913-sense | TCACAAGAAAGTTCGGCTGCTAC | SMU.1913 | SMU.1913-antisense | CTGCTGGCAAATTCGCTTACTTG |
| SMU.1960-sense | ACTGTAACACGTTGGGCGAAAG | SMU.1960 | SMU.1960-antisense | AGCAGCAAAGGCTTCTTTAGACC |