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. 2007 Oct 5;189(23):8564–8574. doi: 10.1128/JB.01121-07

FIG. 5.

FIG. 5.

Expression of R. capsulatus olsA and plsC316 in E. coli. (A) The E. coli plsC(Ts) strain SM2-1 harboring plasmids carrying olsA, plsC316, or plsC3498 of R. capsulatus was grown on 2% l-arabinose-containing LB plates at 42°C to score heterologous complementation. SM2-1 cells carrying the cloning vector pBAD/Myc-His A were used as a control. (B) Expression of R. capsulatus olsA and plsC316 in E. coli plsC(Ts) mutant SM2-1 cells before (0) and after (2) induction with 2% l-arabinose for 4 h at 30°C. Following induction cells were resuspended in 2× SDS loading buffer, and expressed proteins were detected by SDS-PAGE and immunoblotting using anti-Myc antibody as described in Materials and Methods. The triangles point out the R. capsulatus OlsA and PlsC316 proteins (31 and 29.5 kDa, respectively) together with the 32.5- and 25-kDa molecular mass markers.