FIG. 3.
Inactivation of the Eps pathway results in the leakage of periplasmic proteins. Culture supernatants and periplasmic as well as cytoplasmic extracts from cells present in overnight cultures of wild-type TRH7000, TRHΔeps, and PBAD::eps (grown in the absence of arabinose) were assayed for the presence of β-lactamase, AP, or GFP as described in Materials and Methods. The level of each of these proteins in the culture supernatant was expressed as a percentage of the total amount of each protein present in both culture supernatant and cellular extract. The means of four independent experiments ± SEM are presented. For all proteins tested, P < 0.02.