FIG. 7.

Reduced level of OmpU in Δeps mutants. Western blot of cellular extracts obtained from cultures of wild-type, NΔeps, and NΔeps+pEps strains of V. cholerae N16961 grown in LB broth or in LB broth supplemented with glucose (LBG) or sucrose (LBS) to a 5% final concentration for 16 h at 37°C. Samples were matched by equivalent OD₆₀₀ units, separated by SDS-PAGE, transferred to nitrocellulose, and probed with OmpU antiserum.