TABLE 2.
Effect of eps mutation on antimicrobial resistance
| Strain | MIC50a
|
|
|---|---|---|
| Polymyxin B (U/ml) | Bile salts (%) | |
| N16961 | ||
| Wild type | 800 | 0.05 |
| NΔepsc | 100 | 0.0005 |
| NΔeps+pEps | 800 | 0.05 |
| TRH7000 | ||
| Wild type | 400 | NDb |
| TRHΔepsc | 100 | ND |
| PBAD::epsc | 100 | ND |
| TRHΔeps+pEps | 400 | ND |
| PBAD::eps+arabinose | 400 | ND |
| TRHΔepsLc | 100 | ND |
| TRHΔepsL+pEpsL | 400 | ND |
a
The assays were conducted at least three times, and all standard deviations were less than 15%.
b
ND, not determined.
c
Colonies were counted after 48 h of incubation at 37°C.