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Heparin sulfate binding phenotypes of AAV capsids. Slot blot analysis was performed with flowthrough (F), wash 1 (W), wash 2 (Wf), and elution (E) fractions of AAV2, AAV5, and AAV8 capsids following loading onto a heparin-agarose column. The loaded sample is shown in the sample (S) slot. AAV2, positive control; AAV5, negative control.
