Skip to main content
. 2007 Sep 5;81(22):12450–12457. doi: 10.1128/JVI.01470-07

FIG. 3.

FIG. 3.

Interaction of the IVa2 and 22-kDa proteins with mutant A1-A2 probes. (A) Sequences of the wild-type and mutant probes. The bold lines designate the A-repeat consensus (TTTGN8CG), and the gray boxes indicate the TTTG and CG motifs. (B) Autoradiogram of EMSA comparing binding of the 22-kDa protein (22K) in the presence (+) of 10 nM IVa2 protein (IVa2) to the A1-A2 wild-type and mutant probes. Dashes above lanes indicate reactions that did not contain the indicated protein. Triangles above the lanes represent increasing concentrations of the 22-kDa protein (0.3, 1.6, 8, and 40 nM). (C) Autoradiogram of EMSA comparing binding of the purified IVa2 and 22-kDa proteins or nuclear extracts to the wild-type and mutant probes.