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. 2007 Sep 5;81(22):12450–12457. doi: 10.1128/JVI.01470-07

FIG. 4.

FIG. 4.

Formation of multiple complexes by the IVa2 and 22-kDa proteins on the complete packaging sequence. Autoradiogram from an EMSA with the 22-kDa protein (22K), the IVa2 protein (IVa2), and a probe encompassing the entire packaging sequence from nucleotides 200 to 397. Dashes above lanes indicate reactions that do not contain the 22-kDa or IVa2 protein. The labeled bold lines above the lanes indicate the quantities of the IVa2 protein added to reactions. The leftmost triangle indicates increasing concentrations of the 22-kDa protein (2, 4, and 8 nM) in the absence of IVa2. The other two triangles indicate concentrations of the 22-kDa protein (0.1, 0.3, 0.5, 1, 2, 4, and 8 nM) in the presence of IVa2. Free probe is indicated with a labeled arrowhead.