TABLE 1.
Capsid fragment primers
| Primera | Sequence (5′ to 3′)b | Position (nucleotide)c | Polarity |
|---|---|---|---|
| 1207cacc-418 | CAC CGC TCC TGC CGT TGC CCC C | 1252-1270 | Sense |
| 1207cacc-427 | CAC CGG TGA GCA ACT TCT TTT C | 1279-1296 | Sense |
| 1207-528 | CTA TGT GTA GAA CTG GTT GAC CC | 1553-1575 | Antisense |
| 1207-534 | CTA CCC CGC TCC ATT TCC CAT | 1582-1602 | Antisense |
| 1207-541 | GGG CCA TTA TAA CGC ACG TC | 1604-1623 | Antisense |
a
The numbering of the sense primers indicates the nucleotide sequences of the N-terminal (first) norovirus residue contained within a construct generated with a particular primer. The numbering of the antisense primers indicates the nucleotide sequences of the C-terminal (last) norovirus residue contained within a construct generated with a particular primer.
b
Four bases used for directional cloning are shown underlined.
c
The 1207 sequence was assigned accession number DQ975270 in GenBank.