FIG. 2.

Viral growth, replication, and secreted hIL-12 production. Single-step growth analysis of vMyxIL-12 (dashed line and circles) and vMyxgfp (solid line and triangles) was evaluated on BGMK (A), 8484 (B), and RK-13 (C) cells. Cells were infected with virus at an MOI of 3. Cells were harvested at the times indicated, and the titers of infectious virus present at each time point were determined by subsequent infection on BGMK cells. All growth analyses were performed in triplicate. The production of secreted hIL-12 from infected BGMK cells was measured by enzyme-linked immunosorbent assay (D). Cells were infected at the indicated MOI, and the cellular supernatant was collected 48 hpi. RK-13 cells (E) were infected at an MOI of 3, and cell supernatants were collected at the indicated time points.