Skip to main content
. 2007 Sep 12;81(22):12111–12118. doi: 10.1128/JVI.00560-07

FIG. 1.

FIG. 1.

Effects of IFN-α and IFN-γ on NV replication in HG23 cells. (A) Effects of IFN-α and IFN-γ on the expression of NV genome. One-day-old semiconfluent NV replicon-bearing HG23 cells were incubated with various concentrations (units/ml) of IFN-α and IFN-γ for 72 h, and then total RNA was prepared for real-time qRT-PCR to detect the NV genome. The reduction of NV genome by IFNs was calculated by the comparison to that with mock (medium) treatment. Error bars represent standard deviations from at least three independent experiments. RNA levels for cells subjected to a treatment that were significantly reduced (P < 0.05) compared to the RNA level of mock-treated cells are indicated by asterisks. (B) Effect of IFN-γ on the expression of NV ProPol or neomycin phosphotransferase in HG23 cells. One-day-old semiconfluent HG23 cells were incubated with 0 (mock) or 100 units/ml of IFN-γ for 72 h. The cells were then fixed with 100% methanol and stained with antibodies to NV ProPol (top panels) or neomycin phosphotransferase II (bottom panels) and FITC-conjugated secondary antibody. The negative control includes parental Huh-7 cells treated with 100 units/ml of IFN-γ with the same staining.