FIG. 5.

Effect of supplementation of guanosine on NV replication in ribavirin-treated HG23 cells and effect of MPA on NV replication. (A) Effect of ribavirin alone or with guanosine (100 μM) on the NV genome in HG23 cells. One-day-old semiconfluent HG23 cells were incubated with various concentrations of ribavirin ranging from 0 (mock) to 100 μM with (+) or without (−) 100 μM guanosine. After 72 h of incubation, total RNA was prepared for real-time qRT-PCR to detect the NV genome. The reduction of NV genome by the treatments was calculated by comparison to the value for the mock-treated control. RNA levels for cells treated by guanosine and ribavirin that were significantly different (P < 0.05) from the RNA level of cells treated with ribavirin alone are indicated by asterisks. (B) Effect of MPA on NV replication. One-day-old semiconfluent HG23 cells were incubated with various concentrations of MPA ranging from 0 (mock) to 2 μM for 72 h. After the incubation, total RNA was prepared for real-time qRT-PCR to detect the NV genome. The reduction of NV genome in cells subjected to different treatments was calculated through comparison to mock-treated cells. RNA levels for cells subjected to a treatment that were significantly reduced (P < 0.05) compared to the RNA level of mock-treated cells are indicated by asterisks. Error bars represent standard deviations from at least three independent experiments for panels A and B.