FIG. 3.
Determining the anti-NKG2D antibody concentration needed to completely inhibit NKG2D signaling. Two cell lines, IC-21 macrophages and K42 fibroblasts, constitutively expressed NKG2D ligands. (A) To identify the concentration of anti-NKG2D antibody needed to completely inhibit NK cells lysis, LAK cells were incubated with the targets in the presence of the indicated concentration of antibody at an E/T ratio of 20:1 for a 51Cr release assay. (B) E/T ratio titration of LAK cells with IC-21 cells and K42 targets at 50 μg/ml of anti-NKG2D antibody or control.