FIG. 4.

The lack of viral protein accumulation following infection with 17termA is due to translational arrest mediated by phosphorylated eIF2α rather than autophagy-dependent protein degradation. Shown is an autoradiograph of Atg5^−/− and Atg5^+/+ MEFs mock infected or infected with 17termAR or 17termA at a multiplicity of 5 PFU/cell. At 6 and 12 h postinfection, ³⁵S-containing methionine and cysteine were incorporated into newly synthesized proteins for 2 h, harvested, and then visualized via autoradiography.