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Western blot analysis of PK-resistant mouse PrPSc. RML and ME7 prion-infected brain homogenates were prepared as described in Materials and Methods. Brain homogenates were treated with various concentrations of PK and analyzed by SDS-PAGE and Western blotting, using anti-PrP monoclonal antibody 683. Each track was loaded with 25 μg of total protein. Molecular mass markers (kDa) are shown on the left.
