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. 2007 Aug 29;81(22):12119–12127. doi: 10.1128/JVI.01434-07

FIG. 2.

FIG. 2.

Detection of mouse PrP by CDI. (a) Various concentrations of mouse full-length recombinant PrP protein were captured by purified anti-PrP monoclonal antibody V24 on Nunc Maxisorp ELISA plates. Recombinant PrP was detected with 0.25 μg/ml biotinylated anti-PrP monoclonal antibody 245 (▵) or 6H4 (▴). Results shown are time-resolved fluorescence (TRF) values (mean cps ± standard deviations [SD]) for duplicate wells. (b) Various dilutions of RML-infected mouse brain homogenate were captured by purified anti-PrP monoclonal antibody A516 following treatment with (squares) or without (circles) NaPTA and with 0 M (open symbols) or 6 M (closed symbols) GdnHCl, as described in Materials and Methods. Native PrP was detected with 0.25 μg/ml biotinylated anti-PrP monoclonal antibody 245. Results shown are TRF values (mean cps ± SD) for triplicate wells. (c) Various dilutions of uninfected CD-1 mouse brain homogenate were captured by purified anti-PrP monoclonal antibody A516 following treatment with (squares) or without (circles) NaPTA and with 0 M (open symbols) or 6 M (closed symbols) GdnHCl, as described in Materials and Methods. Native PrP was detected with 0.25 μg/ml biotinylated anti-PrP monoclonal antibody 245. Results shown are TRF values (mean cps ± SD) for triplicate wells.