FIG. 4.

Conformational stabilities of RML and ME7 PrP27-30 proteins, measured by direct ELISA. RML (○) and ME7 (•) prion-infected mouse brain homogenates were prepared as described in Materials and Methods. Brain homogenates were treated with various concentrations of GdnHCl, followed by 32 μg/ml of PK, prior to treatment with NaPTA. All samples were denatured using 6 M GdnHCl before inoculation onto ELISA plates. Biotinylated anti-PrP monoclonal antibody 6H4 was used as the detector antibody. Results are shown as percent PrP27-30 for triplicate wells. Data shown represent one of three experiments demonstrating similar trends.