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Western blot analysis of PK-resistant PrPSc conformers. RML and ME7 prion-infected mouse brain homogenates were prepared with Sarkosyl as described in Materials and Methods. Brain homogenates were treated with various concentrations of PK and with or without NaPTA prior to analysis by SDS-PAGE and Western blotting, using anti-PrP monoclonal antibody 683. Each track was loaded with 50 μg of total protein. Molecular mass markers (kDa) are shown on the left.
