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Western blot analysis of Sarkosyl-treated RML and ME7 PrP27-30. RML and ME7 prion-infected mouse brain homogenates were prepared as described in Materials and Methods. Brain homogenates were treated with various concentrations of Sarkosyl in PBS, followed by treatment with or without PK prior to analysis by SDS-PAGE and Western blotting, using anti-PrP monoclonal antibody 683. Each track was loaded with 50 μg of total protein. Molecular mass markers (kDa) are shown on the left.
