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. 2007 Sep 5;81(22):12135–12144. doi: 10.1128/JVI.01296-07

TABLE 3.

Secondary mutations identified in WT (VUSS3) and ExoN mutant viruses

Virus Mutation no.a Mutationb Amino acid changec Locationd
VUSS3p5c2 1 C21149A Pro102Thr nsp16 CDS
VUSS3p5c4 2 A4608G Asn635Asp nsp3 CDS
VUSS3p5c5 3 C21149A Pro102Thr nsp16 CDS
VUSS3p5c6 4 A21515C Met224Leu nsp16 CDS
rExoN1p5c1 5 U150C Silent 5′ UTR
6 G1092A Val48Ile nsp2 CDS
7 G17723A Val455Ile nsp13 CDS
8 A21069U Asp75Val nsp16 CDS
9 U24030C None S gene
10 U25845C None S gene
11 U28207C None ORF4b
rExoN1p5c2 12 U694A Leu162Gln nsp1 CDS
13 U14122C None nsp12 CDS
14 G15133A None nsp12 CDS
15 A15706G None nsp12 CDS
16 A17747G Lys463Glu nsp13 CDS
17 A18802U None nsp14 CDS
18 G19115A Ala319Thr nsp14 CDS
19 U23407A Silent HE
20 U25783A Phe619Ile S gene
21 U26721C None S gene
22 C28194U Thr46Met ORF4b
rExoN3p5c1 23 G812U None nsp1 CDS
24 U2144A None nsp2 CDS
25 U4081G Ile459Ser nsp3 CDS
26 U13481C None nsp10 CDS
27 G15375A Arg600His nsp12 CDS
28 G17711A Val451Met nsp13 CDS
29 U18772C None nsp14 CDS
30 C27928A Silent IGR
31 G27930U Silent IGR
32 A27931C Silent IGR
33 U insertione Multiple ORF4b
rExoN3p5c2 34 A1342G Glu131Gly nsp2 CDS
35 C3337U Ala211Val nsp3 CDS
36 U17227A His289Gln nsp13 CDS
37 G17452A None nsp13 CDS
38 A23139G Silent HE
39 U24188C Val87Ala S gene
a

Numbers refer to secondary mutations depicted in Fig. 5.

b

Nucleotide positions refer to the MHV-A59 complete genome sequence (GenBank accession no. AY910861).

c

Amino acid positions in nsps refer to residues in mature, processed nsps (i.e., the first residue after each viral proteinase cleavage site is renumbered as 1).

d

CDS, coding sequence; UTR, untranslated region; ORF4b, putative 11.7-kDa protein; HE, hemagglutinin-esterase pseudogene (not expressed) in MHV-A59; IGR, intergenic region between spike gene and ORF4.

e

A single U insertion was identified in a run of five consecutive U residues (nt 28104 to 28108) and is predicted to result in a frameshift relative to WT sequence and, consequently, in substitutions of His18Ser and Val19Cys and early termination after amino acid residue 19 in ORF4b.