TABLE 1.
Growth of the recombinant H5N1 and H1N1 influenza viruses
| Recombinant virus | Viral yielda in:
|
Plaque sized | ||
|---|---|---|---|---|
| Eggsb | MDCK cellsc | MDCK-SIAT1 cellsc | ||
| H5N1 viruses | ||||
| VN1203 | 9.4 ± 0.4 | 8.7 ± 0.2 | 8.9 ± 0.3 | 2.8 ± 0.3 |
| VN1203-H274Y | 9.1 ± 0.1 | 9.0 ± 0.2 | 8.8 ± 0.7 | 1.7 ± 0.4* |
| VN1203-N294S | 9.2 ± 0.0 | 8.7 ± 0.1 | 8.7 ± 0.4 | 2.1 ± 0.3 |
| VN1203-E119G | 8.4 ± 0.2 | 6.6 ± 0.4* | 5.4 ± 0.1* | Heterogeneouse |
| VN1203-R292K | 8.0 ± 0.6 | 7.8 ± 0.2* | 7.4 ± 0.1* | Heterogeneous |
| H1N1 viruses | ||||
| PR8 | 10.0 ± 0.4 | 8.4 ± 0.1 | 8.4 ± 0.1 | 0.9 ± 0.1 |
| PR8-H274Y | 8.6 ± 0.2* | 7.8 ± 0.2 | 7.7 ± 0.3 | 0.5 ± 0.1* |
| PR8-N294S | 9.5 ± 0.4 | 8.4 ± 0.1 | 7.8 ± 0.3 | 0.8 ± 0.1 |
| PR8-E119G | 7.1 ± 0.5* | 5.8 ± 1.1* | 4.6 ± 1.5* | Heterogeneous |
| PR8-R292K | 7.0 ± 0.4* | 5.3 ± 1.5* | 4.0 ± 1.9* | Heterogeneous |
a
*, P < 0.05 compared to value for wild-type virus.
b
Values are the log10 EID50 ± SD/ml from three independent determinations.
c
Values are the log10 TCID50 ± SD/ml from three independent determinations.
d
Plaque assays were performed in MDCK cells. Values are mean plaque diameter (mm) ± SD as measured by using the Finescale comparator (Los Angeles, CA).
e
Heterogeneous plaques ranged in size from pinpoints to the size of the recombinant wild-type virus plaques.