FIG. 1.

HSV-infected human PBMC selectively synthesize IL-10 in response to TCR stimulation. Human PBMC were incubated with mock-infected or HSV-infected human fibroblasts, as described in Materials and Methods. PBMC were removed by aspiration and transferred to plates that were untreated or coated with immobilized anti-CD3 antibody to specifically stimulate T cells through the TCR. Forty-eight hours later, supernatant was analyzed for cytokine production in triplicate using an ELISA format. The results from five separate experiments for TNF-α, GM-CSF, IL-4, IL-5, and IL-6 and from eight separate experiments for IL-10 are depicted as mean cytokine levels (left column) and median cytokine levels (right column). *, P < 0.05, Student's t test.