FIG. 4.
Interactions of VACV and HIV-1 in human lymphoid tissues coinfected ex vivo. Tissue blocks were coinfected with VACV and either R5SF-162 or X4LAI.04 or were infected singly with one of these isolates. HIV-1 replication was evaluated based on measurement of the p24 core antigen. VACV replication was evaluated based on measurement of VACV genome equivalents in the culture medium by using real-time PCR. A. Typical kinetics of HIV-1 replication in tissue blocks infected singly with HIV-1 or coinfected with VACV. Each point represents a measurement of the pooled medium bathing 27 blocks. B. Average HIV-1 replication in tissues coinfected with VACV and HIV-1, relative to that in matched tissues infected singly with HIV-1. Data are means ± SEM (error bars) of experiments with tissues from 13 donors infected ex vivo with R5SF-162 and from 6 donors infected ex vivo with X4LAI.04. C. Average VACV replication in tissues coinfected with VACV and HIV-1, relative to that in matched tissues infected singly with VACV. Data are means ± SEM (error bars) of experiments with tissues from 13 donors infected ex vivo with R5SF-162 and from 6 donors infected ex vivo with X4LAI.04.