FIG. 2.

DSB-induced LOH in rhp51Δ cells results from Ch¹⁶ truncations. (A) PFGE analysis of chromosomal DNA from a parental rhp51Δ Ch¹⁶-MGH strain (lane 1) and individual rhp51Δ ade⁺ G418^s his⁻ strains isolated following DSB induction (lanes 2 to 6). (B) Schematic of the distal arm of Ch¹⁶-MGH indicating distances of markers from the break site (scissors). (C) Southern blot analysis of the gel in panel A probed for distal marker “dm1” (SPCC338.14), his3, “dm11” (SPCC61.05), and rad21 (as indicated). (D) High-resolution PFGE analysis of the strains described above. Size markers are shown (M).