. 2007 Aug 27;27(21):7745–7757. doi: 10.1128/MCB.00462-07

TABLE 3.

Pedigree analysis of ade⁺ G418^s his⁻ colonies carrying Ch^x

Daughter 1		Daughter 2		Total no.
Phenotype	Genotype	Phenotype	Genotype	Total no.
ade⁺ G418^shis⁻	Ch^x	Nonviable		15
ade⁺ G418^shis⁻	Ch^x	ade⁺ G418^shis⁻	Ch^x	2
ade⁺ G418^shis⁻	Ch^x	ade⁻ G418^shis⁻	Ch^x	1
ade⁺ G418^shis⁻	Ch^x	ade⁺ G418^shis⁺	Ch¹⁶	1
ade⁺ G418^shis⁻	Ch^x	ade⁻ G418^shis⁻	Absent	1
ade⁺ G418^shis⁻	Ch^x	ade⁺ G418^shis⁺	Crossover	1
ade⁺ G418^shis⁻	Ch^x	ade⁻ G418^shis⁻	Ch¹⁶ + other	1

Analysis for sisters of ade⁺ G418^s his⁻ colonies carrying Ch^x is shown. A total of 869 daughter cells were segregated following HO induction in strain TH1900 on YE5S plates. Marker loss was determined for the colonies formed (phenotype). ade⁺ G418^s his⁻ colonies and sister colonies were subject to PFGE analysis, and the chromosomal organization was determined (genotype).