TABLE 4.
Breakpoint telomere junctions in HR mutantsa
| Genetic background | PCR product | Sequence |
|---|---|---|
| ↓ | ||
| MATa | 1 | agtcgggtttttcttttagtttcagctttccgcaacaaaaattttataaaccctggttttg |
| rad32Δ | 1 | agtcgggtttttcttttagtttcaGGTTACGGTTACGGTTACGGTTACAGGGTTACAGGGG |
| 2 | agtcgggtttttcttttagtttcaGCTTACGGTTACAGGGGTTACGGTTACAGGGTTACGG | |
| 3 | agtcgggtttttcttttagtttcagctttccgcaacaGGTTACAGGTTACAGGGGTTACGG | |
| 4 | agtcgggtttttcttttagtttcagctttccGGTTACAGGGGTTACGGTTACAGGGTTACA | |
| 5 | agtcgggtttttcttttagtttcagctttccgcaacGGTTACAGGTTACGGTTACAGGTTA | |
| rad50Δ | 1 | agtcgggtttttcttttagtttcagctttccgcGGTTACAGGGTTACAGGTTACAGGTTAC |
| 2 | agtcgggtttttcttttagtttcagctttccgGGTTACAGGGTTACGGTTACAGTTACGGT | |
| 3 | agtcgggtttttcttttagtttcagctttccGGTTACAGGTTACAGGTTACGGTTACAGGG | |
| 4 | agtcgggtttttcttttagtttcaGGTTACAGGTTACGGTTACGGTTACACGGTTACAGAG | |
| 5 | agtcgggtttttcttttagtttcGGTTACACGGTTACGGTTACGGTTACAGGTTACAGGGT | |
| nbs1Δ | 1 | agtcgggtttttcttttagtttcagctttccgcaacaGTTACAGGTTACAGGTTACAGGTT |
| 2 | agtcgggtttttcttttagtttcagctttccgcaacaCGGTTACACGGTTACACGGTTACA | |
| 3 | agtcgggtttttcttttagtttcagctttccgcaacaGTTACACGGTTACAGGTTACAGGT | |
| 4 | agtcgggtttttcttttagtttcagctttccgcaacaCGGTTACAGGTTACAGGTTACAGG | |
| 5 | agtcgggtttttcttttagtttcagctttccgcGGTTACAGGTTACACGGTTACAGGGTTA | |
| exo1Δ | 1 | agtcgggtttttettttagtttcagGGGTTACAGGTTACAGGTTACGGTTACGGTTAGGTT |
| 2 | agtcgggtttttcttttagtttcagctttccgcaacaGGGGTTACGGTTACAGGGGTTACG | |
| 3 | agtcgggtttttcttttagtttcagctttccgcaacGGTTACAGGTTACGGGTTACAGGGGTT | |
| 4 | agtcgggtttttcttttagtttcagctttccgdaacaGGGGTTACGGTTACAGGGTTACGG | |
| rad22Δ | 1 | agtcgggtttttcttttagtttGGTTACAGGTTACCGGGTTTACGGTTACAGGGGTTACGG |
| 2 | agtcgggtttttcttttagttCGGTTACAGGTTACAGGGGTTACGGTTACAGGGTACAGGG | |
| 3 | agtcgggtttttcttttagtttcagctttccgcaacGGTTACAGGGGTTACGGTTACAGGT | |
| 4 | agtcgggtttttcttttagtttcagctttccgcaacGGTTACAGTTACAGGGTTACAGGTTA | |
| 5 | agtcgggtttttcttttagtttcagctttccGGTTACAGTTACAGGGTTACGGTTACAGGTT | |
| rhp55Δ | 1 | agtcgggtttttcttttagtttcagctttccgcaacaGTTACGGTTACAGGGTTACACGGT |
| 2 | agtcgggtttttcttttagtttcagctttccgcaacaGTTACGGTTACAGGGGTTACGGTT | |
| 3 | agtcgggtttttcttttagtttcagctttccgcaacGGTTACAGGTTACAGGTTACAGGGT | |
| 4 | agtcgggtttttcttttagtttcagctttccGGTTACACGGTTACAGGTTACAGGGGGTAC | |
| 5 | agtcgggtttttcttttagtttCACGGTTACACGGTTACAGGTTACGGTTACAGGTTACGG | |
| rhp51Δ | 1 | agtcgggtttttcttttagtttcagctttccgcaacaGGTTACAGGTTACAGGTTACAGGT |
| 2 | agtcgggtttttcttttagtttcagctttccgcaacGGTTACAGGGTACGGTTACAGGGTT | |
| 3 | agtcgggtttttcttttagtttcagctttccgcaacGGTTACAGGTTACAGGTTACAGGGT | |
| 4 | agtcgggtttttcttttagtttcagctttccGGTTACAGGGTTACAGGGGGTTACGGTTAC | |
| 5 | agtcgggtttttcttttagtttcagctttccGGTTACAGGTTACAGGTTACGGTTACAGGG | |
| rhp54Δ | 1 | agtcgggtttttcttttagtttcagctttccgcaacaGGGTTACGGTTACACGGTACAGGG |
| 2 | agtcgggtttttcttttagtttcagctttccgcaacaGGGTTACGGTTACAGGTTACAGGG | |
| 3 | agtcgggtttttcttttagtttcagctttccgcaacGGTTACGGTTACGGTTACGGTTACG | |
| 4 | agtcgggtttttcttttagtttcagctttccgcaacGGTTACAGGGTTACGGTTACAGGGT | |
| 5 | agtcgggtttttcttttaCGGTTACAGGGTACGGTTACGGTTACAGTTACGGTTACAGGGG | |
| mus81Δ | 1 | agtcgggtttttcttttagtttcagctttccgcaacaGGGTTACAGGTTACAGTTACAGGG |
| 2 | agtcgggtttttcttttagtttcagctttccgcaacaCGGTTACAGGTTACAGGTTACGGT |
a
Examples of breakpoint telomere junctions in HR mutants are listed. Sequences of PCR products acquired from individual TEL+ strains from each background are shown; 61 bp of MATa are depicted, together with the HO endonuclease cleavage site (arrow). The telomeric repeat sequence (G1-5TTACA0-1) is in uppercase letters.