TABLE 5.
Effect of rhp51 overexpression on DSB-induced marker loss in rhp55Δa
| Genetic background | % ade+ G418s/Hygshis+ (GC) | % ade+ G418r/Hygrhis+ (EJ/SCR) | % ade− G418s/Hygshis− (Ch16 loss) | % ade+ G418r/Hygrhis− (LOH) | P value (relative to rhp55Δ LOH) |
|---|---|---|---|---|---|
| rhp55Δ + pIRT3 | 3.1 ± 1.1 | 46.2 ± 3.5 | 45.3 ± 4.6 | 1.1 ± 0.3 | 1.000 |
| rhp55Δ + pIRT3-rhp51 | 12.2 ± 0.2 | 71.5 ± 0.4 | 15.4 ± 0.3 | 0.5 ± 0.1 | 0.166 |
a
Levels of interchromosomal gene conversion (GC), end joining/sister chromatid recombination (EJ/SCR), minichromosome loss (Ch16 loss), and LOH were calculated as described in Materials and Methods.