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. 2007 Sep 4;27(21):7718–7726. doi: 10.1128/MCB.00180-07

FIG. 2.

FIG. 2.

Expression kinetics of EWI-2 on DCs. (a) Expression of EWI-2 and CD86 was analyzed on MoDCs after LPS-plus-IFN-γ stimulation (time = 0 h) by real-time quantitative RT-PCR at different time points. The expression level relative to EF-1α (EF-1α expression level, 1,000) is given. (b) In parallel, the surface expression levels of both molecules were measured by FACS (mean fluorescence intensity [MFI]). (c to e) Changes in cell surface EWI-2 and CD28 were directly compared by two-color FACS at three time points.