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Engaging EWI-2 on DCs diminishes antigen-driven proliferation. DCs were pulsed with 50 μg/ml Der p1 antigen peptide, washed, and placed into culture with 105 responder T cells of the clone 4:3.1. Various concentrations of recombinant HSPA8 or 10 μg/ml anti-EWI-2 mAb were added. Proliferations of responder T cells were determined 3 days later. Two independent donors (a and b) are shown; ★ indicates a P value of <0.05 in comparison to the medium control value.
