FIG. 3.

Hybridization analysis of pre-rRNA processing in cells expressing Nog1G224A. (A) D1106 (wild-type [WT]Nog1) and D1411 (mutant) cells were cultured with (+) or without (−) IPTG for the times indicated. Steady-state levels of pre-rRNAs were analyzed by Northern hybridization of total RNA with the oligonucleotide probes indicated on the left. Pre-rRNAs detected with each probe are indicated on the right. (B) Induction of G224A in cells used for the RNA analysis was assessed by detection with anti-HA antibodies in Western blotting of cell lysates normalized for protein content. (C) Relative positions of probes used in the experiment depicted in panel A on the primary pre-rRNA transcript and the structure of processing intermediates detected with these probes.