TABLE 1.
S. cerevisiae strains and recombinant plasmids used in the present study
| Strain | Genotype | Source or reference |
|---|---|---|
| RSY299 | MATα his3 leu2 trp1 ura3 | 19, 21 |
| RSY302 | MATα leu2 trp1 his3 1 ura3 cdc7-7 | 19, 21 |
| RSY847 | MATα leu2 trp1 his3 1 ura3 cdc7-7 mcm5-P83L (mcm5-bob1-2) | 19, 21 |
| RSY864 | MATα ura3 leu2 trp1 his3 mcm4-P265L | This study |
| RSY855 | MATα leu2 trp1 his3 1 ura3 cdc7-7 mcm4-P265L | This study |
| RSY877 | MATα leu2 ura3 trp1 his7 mcm2-P264L | This study |
| RSY878 | MATα leu2 ura3 trp1 his3Δ-1 cdc7-7 mcm2-P264L | This study |
| RSY1203 | MATaura3 leu2 trp1 mcm2-P264L mcm4-P265L | This study |
| RSY1210 | MATacdc7-7 ura3 leu2 mcm2-P264L mcm4-P265 | This study |
| RSY1216 | MATaura3 leu2 trp1 mcm2-P264L mcm4-P265L mcm5-P83L | This study |
| RSY1217 | MATacdc7-7 ura3 leu2 mcm2-P264L mcm4-P265L | This study |
| RSY902 | MATα cdc7-7 leu2 trp1 his7 ura3 mcm5Δ::kanMX4 (pRS424 2μm MCM5+TRP1) | This study |
| YRL123 | MATα cdc7-7 leu2 trp1 his7 ura3 mcm5Δ::kanMX4 (pRS424 2μm mcm5-P83L TRP1) | This study |
| YRL124 | MATα cdc7-7 leu2 trp1 his7 ura3 mcm5Δ::kanMX4 (pRS424 2μm mcm5-P83A TRP1) | This study |
| YRL125 | MATα cdc7-7 leu2 trp1 his7 ura3 mcm5Δ::kanMX4 (pRS424 2μm mcm5-I159E TRP1) | This study |
| YRL126 | MATα cdc7-7 leu2 trp1 his7 ura3 mcm5Δ::kanMX4 (pRS424 2μm mcm5-I159R TRP1) | This study |
| YRL127 | MATα cdc7-7 leu2 trp1 his7 ura3 mcm5Δ::kanMX4 (pRS424 2μm mcm5-I159A TRP1) | This study |
| YRL136 | MATα cdc7-7 leu2 trp1 his7 ura3 mcm5Δ::kanMX4 (pRS424 2μm mcm5-P83L I159A TRP1) | This study |
| YRL137 | MATα cdc7-7 leu2 trp1 his7 ura3 mcm5Δ::kanMX4 (pRS424 2μm mcm5-P83L I159E TRP1) | This study |
| YRL138 | MATα cdc7-7 leu2 trp1 his7 ura3 mcm5Δ::kanMX4 (pRS424 2μm mcm5-P83L I159R TRP1) | This study |
| YRL154 | MATaleu2 ura3 tyr1 ade1 ade2 trp1 mcm5Δ::kanMX4 (YCp50 2μm MCM5+) | This study |
| RSY311 | MATatrp1 leu2 ura3 can1 his6 bar1 | 40 |
| RSY727 | MATabar1 his6 mcm5-bob1-1 cdc7Δ::HIS3 leu2 ura3 lys2 his3-Δ1 cyh2 | 40 |
| RSY728 | MATabar1 his6 mcm5-bob1-1 trp1 leu2 ura3 lys2 his3-Δ1 | 40 |
| RSY466 | MATaleu2 ura3 trp1 his7 | 32, 40 |
| P253 | MATaura3 can1 trp1 his3 cdc7-1 mcm5-bob1-1 cyh2 | 19 |