TABLE 2.

ARS CEN plasmids replicate poorly in bypass mode

Plasmida	Origin	% Coloniesb at:
		22°C	36°C
pUN70	ARS1	60	0.2
YCp50	ARS1	64	0.3
pRS424	2μm	64	29
pRS314	ARSH4	65	0.1
pYES2	2μm	91	69

^aAll plasmids are in cdc7^ts mcm5-bob1 strain P253. Strains were grown in selective medium at 22°C and then diluted and plated on either selective or nonselective media at 22 or 36°C and incubated for 2 to 3 days.

^bThe numbers are the percentages of the colonies found on selective solid media compared to nonselective media.