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. 2007 Aug 27;27(21):7683–7692. doi: 10.1128/MCB.00577-07

FIG. 7.

FIG. 7.

Reduced expression of Cx43 in Smad4-deficient ECs. (A) Immunofluorescence labeling of Cx43 in PmT-transformed ECs. The Smad4-deficient ECs demonstrate notably reduced expression. (B) Representative RT-PCR analysis of Cx43 expression using RNA extracts from isolated Tie2-positive cells (left side) and PmT-transformed ECs (right side). (C) Western blotting analysis of Cx43 expression in PmT-transformed ECs. “+T” indicates stimulated by 10 ng of TGF-β1/ml for 20 h. The mutant ECs fail to upregulate the expression of Cx43 upon treatment of TGF-β1. (D) Cx43 Immunofluorescence staining of E9.5 DAs shows reduced expression in the mutant vascular ECs in vivo. Scale bar: 12 μm (A) or 25 μm (D).