TABLE 1.

Scansite and NetPhos scores for IKKβ substrates^a

IKKβ substrate	Site	Scansite score	NetPhos score
IκBα	S32	0.552	0.995
	S36	0.575	0.998
RelA (p65)	S536	0.513	0.962
NEMO	S376	1.047	0.008
FOXO3a	S644	0.670	0.017
IRS-1	S312	1.113	0.919
BCL-10	S134	0.950	0.926
	S136	0.719	0.987
	S138	0.662	0.995
	S141	0.757	0.922
	S144	0.711	0.915
Dok1	S439	1.217	0.179
	S443	0.938	0.304
	S446	1.127	0.030
	S450	0.889	0.013
A20	S381	1.043	0.973

^aScansite scores for each protein were determined using a matrix created from IKKβ specificity values (data not shown). A lower score represents a better match to the optimal motif. NetPhos provides scores ranging from 0 to 1. Residues receiving NetPhos scores above 0.5 are considered potential phosphorylation sites, with higher scores representing the most likely sites of phosphorylation. A20 S381 received Scansite and NetPhos scores comparable to those of known IKKβ phosphorylation sites (10, 13, 15, 19, 20, 26, 27, 33).