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. 2007 Aug 27;27(21):7582–7593. doi: 10.1128/MCB.00493-07

FIG. 2.

FIG. 2.

Thrombin-mediated Ca2+ signaling stimulates mROS generation. MPMVECs loaded with Fluo-4/AM (green) and the mROS indicator MitoSOX Red (red) were stimulated with 500 mU/ml thrombin. (A) Live imaging of thrombin-evoked cytosolic Ca2+ mobilization and mROS production. (B to E) Representative tracings of cytosolic Ca2+ and mROS generation in response to 500 mU/ml (B) or 1 mU/ml (C) thrombin, 500 mU/ml thrombin plus 2 U/ml Hir (D), or 10 μM Iono (E). (F) Thrombin-induced mROS production in response to 500 mU/ml thrombin in MPMVECs pretreated with RR (10 μM or 20 μM). (G and H) mROS generation by thrombin in gp91phox−/− MPMVECs (G) or in MnSOD-overexpressing WT MPMVECs (H). (I) Quantitation of peak MitoSOX Red fluorescence following thrombin or Iono addition.