FIG. 2.

RAD5 and RAD52 control alternate pathways for postreplication repair of UV-damaged DNA. Sedimentation in alkaline sucrose gradients of nuclear DNA from cells incubated for different periods following UV irradiation with 3.5 J/m². The rad1Δ rad5Δ (A) and rad1Δ rad5Δ rad52Δ (B) strains were UV irradiated at 3.5 J/m² and then pulse-labeled with [³H]uracil for 15 min, followed by a 30-min (Δ) or 6-h (•) chase in high-uracil medium. Synthesis of normal-size DNA from unirradiated cells pulse-labeled with [³H]uracil for 15 min was followed by a 6-h chase (○). The data for the rad1Δ rad5Δ strain in panel A shown here for comparison are taken from reference 9.