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. 2007 Sep 4;27(21):7615–7622. doi: 10.1128/MCB.01239-07

FIG. 2.

FIG. 2.

Skp2B but not Skp2A overexpression leads to a reduction in REA and activation of the ER. (A) Western analysis of the levels of REA and Skp2B in MCF-7 cells, MCF-7-Skp2B cells, and MCF-7 cells transfected with siRNA against Skp2B (siSkp2B). (B) The reduction of Skp2B mRNA following the transfection of siRNA against SKP2B was determined by quantitative RT-PCR. (C) MCF-Skp2B cells were transfected with FLAG-tagged dominant negative cul-1 (dn Cul-1-F), and the levels of dn-Cul-1-F and REA were determined by immunoblotting (IB). (D) Immunoblot analysis of the levels of REA and Skp2A in MCF-7 and MCF7-Skp2A cells. (E) ER activity was monitored using an ERE luciferase reporter transfected in MCF-7, MCF-7-Skp2B, and MCF-7 cells cotransfected with siRNA Skp2B. (F) Immunofluorescence of REA in MCF-7 and MCF-7-Skp2B cells.